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Chromatography


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Chromatography
A technique for separating the components of a mixture – for example different food colouring agents.
Paper chromatography
Chromatography carried out by spotting drops of the samples onto paper, and then allowing a solvent to move up the paper. Different components in the samples travel up the paper in the solvent at different rates.
Stationary phase
The surface through which the solvent and dissolved substances move in chromatography.
Mobile phase
In paper chromatography, the solvent that moves along the paper carrying the dissolved samples with it.
Chromatogram
The piece of paper showing the results of carrying out chromatography on substances.
Rf value
The ratio of the distance travelled by the solute on a chromatogram (measured from the centre of the spot) to the distance travelled by the solvent under the same conditions. The values for different substances can be used to identify them.